Remove the Etherchannel on your Cisco switch and ask your self what kind of problem you would solve with the etherchannel.
First, I agree with the other comment: etherchannels can cause issues when used for the wrong reasons.
But back to your issues. When you installed ESXi did you configure an IP address and other networking parameters i.e. gateway? After you configure that you would have a Standard Switch with a port group for your vmkernel adapter vmk0. This adapter would have the IP config that you configured during setup. If things are not working open the console of your server and login to the DCUI (Direct Console UI).
You can also compare settings with the second host.
I was able to gain access to the DCUI where I configured the settings identical to Host #2. It was finally narrowed down that the portchannel was the issue.
The other host has VLAN 400 and is able to communicate on the network which is confusing. I had to remove VLAN 400 inside of the new ESXi in order to get connectivity. I am not sure if it is due to me not having the networking portion configured yet in the host (portchannels, vmkernels, vnics, etc.).
Finally, I read that I can export the configuration from Host #2 for backup. Is it possible to tweak that configuration file (change IP address and other information) and import it into the new Host seeing that both host would have nearly the same information (portchannels, vmkernels, vnics, etc)? I am trying to cut down on time but ensure I am configuring it correctly. I was not sure I could do that or not.
Thanks for any additional information you both can provide.
Afaik there is no option to export a configuration from a host and import it to another besides Host Profiles. But even then, a host profile for a host is always unique (IP address etc.) Make sure that the ports the hosts are connected to, are trunk ports on your switch and configure the correct VLANs on those ports. Also, make sure that the correct VLAN is configured for your management network (DCUI). Other VLAN can be used for the port groups.