On test windows server 2016 work correctly, I think...production Connection working on 2008R2, but in Windows noboby turned on TLS1.2...
Does Connection server use the Windows encryption library?
Create same test enviromen, all work perfectly. Attempt reinstall COnnection server
Is this a persistent VM? If so can you remove it from Horizon View shut it down and re-add it to Horizon View and let Horizon View power it on.