Currently running vSphere 5.5 which will be upgraded to 6.0 over the weekend. It is currently booting from a local datastore on the host but is it possible to move this to boot from USB or will a fresh install on the USB be required?
You will need a fresh install.
But you can make a host profile before the reinstall, then install 5.5 to the usb, apply the host profile and then finally upgrade to esxi 6.
In recent times times I have started using dhcp for all my esxi hosts which makes rebuilding or deploying new very easy. You just need a dhcp reservation and forward and reverse dns entries. Iscsi, v motion interfaces don't need static entries.
You will need a fresh install.
But you can make a host profile before the reinstall, then install 5.5 to the usb, apply the host profile and then finally upgrade to esxi 6.
In recent times times I have started using dhcp for all my esxi hosts which makes rebuilding or deploying new very easy. You just need a dhcp reservation and forward and reverse dns entries. Iscsi, v motion interfaces don't need static entries.