'Access Control' Encryption via Workstation is an outer layer of encryption. Removing that layer, in itself, won't alter the contents (aside from the actual decryption of existing data back to a standard 'unencrypted' format) of the VM. As for removing 'this' disk or 'that' disk or editing this preference etc..I cannot say as I do not know your VMs layout or setup internally. I suppose if you are worried about it or do run into issues you could start by disabling SecureBoot inside the VM before doing the rest. Good Luck!
