I recently went through this with Windows 7. MY environment is on 7.3.2 and we have multiple domain controllers. Have you checked all your domain controllers event viewer for failures to authenticate? My environment would start the day working perfectly, and as users logged in and out through the day. Issues related to GPO not applying and UEM settings not being applied started happening. Without much help from VMware, we found the errors in the image on our domain controllers. Along with a Windows patch related to vmxnet3 NIC's, we followed the KB below and pointed the clones to one specific DC. Restarted the connection server and things have been smooth since.
Windows hotfix: Windows6.1-KB2550978-x86
