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Network packets sizes are defined by the end devices. For software iSCSI that's the ESXi host VMkernel port groups on one side, and the storage system on the other side. All switches/devices between them have to be able to handle the sender's packet size. With Jumbo frames configured (usually 9000 or 9216 on some switches), all network packets with this size, or smaller will be transferred to the destination without the need to fragment them. I usually only set MTU 9000 for iSCSI traffic.
André