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Hi @KarinB ,
vCenter is a management server and does not enable connectivity between all host NICs. It's a configuration on hosts that is required to enable communication between them. As far as vMotion network is concerned, yes you need routing to enable connectivity between vMotion networks in different subnets.
To begin with, you can initiate vmkping from source vMotion vmkernel to target vmkernel --> https://kb.vmware.com/s/article/1003728
Hope that helps