Need suggestions and advice's, as we looking to upgrade vcenter from 5.5u3 (running in Windows 2008) to 6.5u1 (looking for VCSA). Please let me know the what actions we need to take care before upgrading the same.
You could also check the links mentioned below, which has the step by step process of the migration from a windows VC to VCSA.
How to upgrade Windows vCenter 5.5 to the 6.5 Appliance - VirtuallyBoring
First go through VMware Interoperablility for current and latest version.
VMware Product Interoperability Matrices
Kindly refer below link for tour reference for upgradation best practice:-
https://kb.vmware.com/s/article/2146439
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Great articles. So are these migrated with the migration-assistant Custom roles and permissions, Folders, resource pools, compute/datastore clusters
Yes, if you're using the migration path built into the vCSA, but not if you do a "swing" migration by standing up an independent vCSA and manually moving hosts.