Hello,
When we are doing OU based assignments for instant clone VDI desktops, its important to use naming prefix of VDI desktop naming pattern at "Limit delivery for these assignments" option. This is a check box is available during assignment of application.
If we dont use this, it will assign application to all the computers in that OU resulting that even internal "it-xxxxxxxx" (Instant Clone Internal Template) Computer object getting the application assignment. Since this is hot-add may interrupt the forking process.
For more information please refer this link: Application Assignment to Specific Computers
