Did you try

i tired vmkchdev -v 000:001:00.1

or rather vmkchdev -v 000:001:00.01 ?

Besides that ... I can't check on a test system at the moment ... so ...

After the vmkchdev -v  did you do a vim-cmd hostsvc/net/refresh  ?

I think a reboot is needed to get the functionality back ... Unless you configured it to be persistent?

I'm not sure if this applies to 4.1 but ..on 5.0 we can run :

# esxcfg-vswitch -l
Switch Name      Num Ports   Used Ports  Configured Ports  MTU     Uplinks
vSwitch0         128         4           128               1500    vmnic0

  PortGroup Name        VLAN ID  Used Ports  Uplinks
  VM Network            0        1           vmnic0
  Management Network    0        1           vmnic0

Switch Name      Num Ports   Used Ports  Configured Ports  MTU     Uplinks
vSwitch1         128         6           128               1500    vmnic2,vmnic3

  PortGroup Name        VLAN ID  Used Ports  Uplinks
  VSANET/vMotion        0        1           vmnic2,vmnic3
  VMkernel for iSCSI/vMotion II  0        1           vmnic3
  VMkernel for iSCSI/vMotion I  0        1           vmnic2

and

# esxcfg-vmknic -l
Interface  Port Group/DVPort   IP Family IP Address                              Netmask         Broadcast       MAC Address       MTU     TSO MSS   Enabled Type
vmk0       Management Network  IPv4      16.87.157.68                            255.255.252.0   16.87.159.255   00:17:a4:77:0c:1c 1500    65535     true    STATIC
vmk1       VMkernel for iSCSI/vMotion IIPv4      0.0.0.0                                 0.0.0.0         0.0.0.0         00:50:56:73:5b:81 1500    65535     true    DHCP
vmk2       VMkernel for iSCSI/vMotion IIIPv4      169.254.0.1                             255.255.0.0     169.254.255.255 00:50:56:77:f9:e9 1500    65535     true    DHCP

and you can disable a vmk ...

#  esxcfg-vmknic -h
esxcfg-vmknic <options>
-a|--add                      Add a VMkernel NIC to the system,
                              requires IP parameters and
                              connection point(portgroup/dvs-name & dvport-id).
-d|--del                      Delete VMkernel NIC on the system
                              requires connection point(portgroup/dvs-name & dvport-id).
-e|--enable                   Enable the given NIC if disabled.
-D|--disable                  Disable the given NIC if enabled.
-p|--portgroup                Setting portgroup as connection point.
-s|--dvs-name                 Setting DVSwitch name of the connection point.
                              This requires --dvport-id option to
                              be given in the same command.
-v|--dvport-id                Setting DVPort ID of connection point.
                              This requires --dvs-name option to
                              be given in the same command.
-l|--list                     List VMkernel NICs.
-i|--ip <IP Spec>             Where <IP Spec> can be one of the following...
                              <X.X.X.X>      - to use static IPv4 address specified
                              DHCP           - to enable IPv4 DHCP for address.
                              <X:X:X::/X>    - to use static IPv6 address
                              DHCPV6         - to enable IPv6 DHCP for address.
                              AUTOCONF       - to enable IPv6 Router advertised address.
-U|--unset-ip <IP Spec>       Where <IP Spec> can be one of the following...
                              V4             - to unset IPv4 address.
                              <X:X:X::/X>    - to remove the specified IPv6 address
                              DHCPV6         - to disable IPv6 DHCP address.
                              AUTOCONF       - to disable IPv6 Router advertised flag.
-n|--netmask <X.X.X.X>        The IP netmask for this VMkernel NIC.
                              Setting the IP netmask requires that the --ip
                              option be given in the same command.
-P|--peerdns                  Set peer dns. If set the system
                              will use the HostName, HostIPAddress
                              Domain returned by DHCP. Valid only for DHCP
-m|--mtu MTU                  MTU for the interface being created.
                              vmknic being created.
                              Valid MTU values are 1280-9000
-6|--enable-ipv6 <true|false> Enable or disable IPv6 for next boot.
-r|--restore                  Restore VMkernel TCP/IP interfaces from
                              Configuration file (FOR INTERNAL USE ONLY).
-h|--help                     Show this message.

Examples:

  To add a vmknic to a portgroup
      esxcfg-vmknic -a -i DHCP -p pgName
      or
      esxcfg-vmknic -a -i x.x.x.x -n 255.255.255.0 pgName

  To delete a vmknic from a portgroup
      esxcfg-vmknic -d -p pgName
      or
      esxcfg-vmknic -d pgName

hope that helps ..

Hello IT. Did you switch it off and on again?