No but I did run into a problem with the first release of the W2K post SP4 Roll-up patch. It blue screened a couple of my servers, but it was not VMware related.
I haven't seen many problems yet with any of the patches. Like above - had some problems with citrix and the 2K rollup (Really should have been SP5). The rollup SP4 patch also has a problem with dynamic disk on W2K servers (thanks to basic disks on vm's - no problem).
We rolled this patch out to 40 Windows 2000 VMs and over 200 physical servers on Friday, no problems reported as of yet. I would be interested in what you are seeing.